|- cells were directly lysed in culture flasks with RNA-Bee to avoid changes in expression due to harvesting or washing.
- Adjust buffers to pH to 6.5 - 7.5 if they have have an acidic pH for better results.
|- An additional wash with 75% ethanol improves 260/280 ratio
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2 years ago
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
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