TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was purified via a hybrid protocol using TRIzol (Invitrogen, Carlsbad, CA) and the RNeasy Cleanup Kit (Qiagen, Valencia, CA). TRIzol was added directly to cells in culture and gently collected and lysed using a cell lifter. RNA extraction was carried out per manufacturer's instructions until addition of ethanol. Following this step, the preparation was added to the RNeasy Mini Spin Column (Qiagen) and purified per manufacturer instructions with on-column DNase digestion. RNA was eluted in 100 μ l of RNase-free water and purity and concentration was assessed with microanalysis (Agilent Bioanalyzer 2100). RNA samples were stored at −80°C until microarray analysis.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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