ApoAlert™ DNA Fragmentation Assay Kit

TUNEL assay cell type - HeLa cells human cervical cancer

Experiment
TUNEL assay cell type - HeLa cells human cervical cancer
Product
ApoAlert™ DNA Fragmentation Assay Kit from Takara Bio Inc
Manufacturer
Takara Bio Inc

Protocol tips

Upstream tips
If stored at –20°C, 20X SSC must be warmed to room temperature to ensure that all salts are in solution prior to 10X dilution with deionized H2O.
After opening the kit, 20X SSC can be stored at room temperature.
Protocol tips
You must separate Coplin jars that have been used with DNase from DNase-free Coplin jars.

Publication protocol

Apoptotic cell death was measured four hours after the addition of Tet by the deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method, using ApoAlert DNA fragmentation assay kit (Clontech Europe, France), and according to the manufacturer's protocol. Images were analyzed using a Zeiss immunofluorescence microscope.

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Reviews

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

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Manufacturer protocol

Download the product protocol from Takara Bio Inc for ApoAlert™ DNA Fragmentation Assay Kit below.

Download PDF Download manufacturer protocol

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