TRIzol Reagent

RNA isolation / purification Cells - immortalized BxPC-3

Experiment
RNA isolation / purification Cells - immortalized BxPC-3
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was extracted from BxPC-3 and BxPC-3-LN cells, respectively, with TRIzol (Life Technologies, USA) and dissolved in Milli-Q H2O. The RNA from BxPC-3 was labeled with Cy3-dUTP and that from BxPC-3-LN with Cy5-dUTP. Fluorescent probe mixtures were denatured at 95°C for 5 min, and applied to the prehybridized chip (Affymetrix, Inc., USA) under a cover glass, which was hybridized at 42°C for 15–20 h. After washing, the microarray was scanned on a scanner (Agilent, G2565BA) and the image was analyzed using Feature Extraction software (Agilent). The signal intensity of each spot was calibrated by subtraction from the intensity of the negative control. The assay was performed twice and only genes showing the same tendency of differential expression were eligible for the final-result analysis.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized BxPC-3 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Development of a unique mouse model for pancreatic cancer lymphatic metastasis.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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