TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was extracted from BxPC-3 and BxPC-3-LN cells, respectively, with TRIzol (Life Technologies, USA) and dissolved in Milli-Q H2O. The RNA from BxPC-3 was labeled with Cy3-dUTP and that from BxPC-3-LN with Cy5-dUTP. Fluorescent probe mixtures were denatured at 95°C for 5 min, and applied to the prehybridized chip (Affymetrix, Inc., USA) under a cover glass, which was hybridized at 42°C for 15–20 h. After washing, the microarray was scanned on a scanner (Agilent, G2565BA) and the image was analyzed using Feature Extraction software (Agilent). The signal intensity of each spot was calibrated by subtraction from the intensity of the negative control. The assay was performed twice and only genes showing the same tendency of differential expression were eligible for the final-result analysis.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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