RNeasy Plus Mini Kit

RNA isolation / purification Cells - immortalized Caco-2

Experiment
RNA isolation / purification Cells - immortalized Caco-2
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

For the gene expression experiments, 2.5 × 105 and 0.9 × 105 Caco-2 cells were seeded in 2 mL complete cell culture medium, in a 6 well/plate (Falcon, Italy), and incubated for 24 and 72 h with 5 nm or 30 nm AuNPs, at either 100 and 300 μM concentrations. At the end of the treatment, the exposure medium was removed and cells were washed twice with PBS, and harvested with 300 μL of RLT plus RNeasy lysis buffer (Qiagen, USA). Cell lysates were collected from wells and stored at −80 °C until the RNA extraction step was performed.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Caco-2 using RNeasy Plus Mini Kit from Qiagen.

Paper title
Changes in Caco-2 cells transcriptome profiles upon exposure to gold nanoparticles.
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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