RNeasy Plus Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

For the gene expression experiments, 2.5 × 105 and 0.9 × 105 Caco-2 cells were seeded in 2 mL complete cell culture medium, in a 6 well/plate (Falcon, Italy), and incubated for 24 and 72 h with 5 nm or 30 nm AuNPs, at either 100 and 300 μM concentrations. At the end of the treatment, the exposure medium was removed and cells were washed twice with PBS, and harvested with 300 μL of RLT plus RNeasy lysis buffer (Qiagen, USA). Cell lysates were collected from wells and stored at −80 °C until the RNA extraction step was performed.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

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