NucleoSpin® RNA

RNA isolation / purification Cells - immortalized Caco-2

Experiment
RNA isolation / purification Cells - immortalized Caco-2
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

RNA was isolated from Caco-2 cells using a NucleoSpin RNA II mini spin kit (TaKaRa Bio), and PCR was performed to confirm that no genomic DNA contamination had occurred.Human small intestine RNA was purchased from Agilent Technologies (Santa Clara, CA, U.S.A.). cDNA synthesis from total RNA (1 µg) was performed by the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) with random primers. qPCR was performed using the KAPA SYBR Fast qPCR Kit (KAPA Biosystems, Boston, MA, U.S.A.) and the Eco Real-Time PCR System (Illumina Inc., San Diego, CA, U.S.A.). The CES mRNA expression levels were normalized by the glycelaldehyde-3-phosphadte dehydrogenase (GAPDH) mRNA level. RT-PCR was also performed using the KAPA taq DNA polymerase (KAPA Biosystems)

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Caco-2 using NucleoSpin® RNA from Macherey Nagel.

Paper title
Development of a Caco-2 Cell Line Carrying the Human Intestine-Type CES Expression Profile as a Promising Tool for Ester-Containing Drug Permeability Studies.
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Manufacturer protocol

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