TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

The tissue sample was grinded in pre-chilled mortars with liquid nitrogen. TRIzol reagent (1 ml per 50-100 mg) was added when homogenizing. Then, the powders were transferred to 2-ml or 1.5-ml microcentrifuge tubes. The cultured cells were lysed directly in the dish with 0.3-0.4 ml of TRIzol reagent per 1 × 105-107 cells. Then, RNA was isolated from harvested cells, xenograft tumors, or human tissues with TRIzol reagent according to the manufacturer’s instructions (Invitrogen, CA, USA). Real-time PCR reactions were performed using SYBR Premix DimerEraser (Takara, Dalian, China), and human GAPDH was used as an endogenous control for mRNA detection. The expression of each gene was quantified by measuring Ct values and normalized using the 2-ΔΔct method relative to GAPDH

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Cal-27 using TRIzol Reagent from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized Cal-27 using TRIzol Reagent from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.