TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was isolated from cells and tissues using the TRIzol Reagent (Invitrogen, Paisley, UK) according to the manufacturer's instructions. All isolated RNA samples had an A260 nm/A280 nm ratio >1.8, as measured by spectrophotometric analysis in 10 mM Tris/HCl buffer, pH 7.5. The concentration of total RNA was determined by a fluorometric assay using the RiboGreen RNA Quantitation Kit (Molecular Probes, Eugene, OR, USA) and the manufacturer's protocol.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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