TRIzol Reagent

RNA isolation / purification Cells - immortalized COR-L23

Experiment
RNA isolation / purification Cells - immortalized COR-L23
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was isolated from cells and tissues using the TRIzol Reagent (Invitrogen, Paisley, UK) according to the manufacturer's instructions. All isolated RNA samples had an A260 nm/A280 nm ratio >1.8, as measured by spectrophotometric analysis in 10 mM Tris/HCl buffer, pH 7.5. The concentration of total RNA was determined by a fluorometric assay using the RiboGreen RNA Quantitation Kit (Molecular Probes, Eugene, OR, USA) and the manufacturer's protocol.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized COR-L23 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Down-regulated expression of apoptosis-associated genes APIP and UACA in non-small cell lung carcinoma.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

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