RNeasy Micro Kit

RNA isolation / purification Cells - immortalized COV-434

Experiment
RNA isolation / purification Cells - immortalized COV-434
Product
RNeasy Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer.

- When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

Total RNA from cells and tumor tissues were extracted using RNeasy Micro or Mini Kit (Qiagen) according to the manufacturer’s instructions cDNA synthesis from total RNA was performed using High Capacity RNA to cDNA Master Mix (Applied Biosystems) in accordance with the manufactures instructions. Real time quantitative PCR was performed using an Applied Biosystems Prism 7500 or Step-One Plus sequence detection system and data were analyzed according to the ΔΔCT method and expressed relative to the control mean (set to equal “1”) as described previously

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized COV-434 using RNeasy Micro Kit from Qiagen.

Paper title
BMP- specific SMADs function as novel repressors of and modulate its expression in ovarian granulosa cells and tumors
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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