HiPurA™ Total RNA Miniprep Purification Kit

RNA isolation / purification Cells - immortalized HaCaT

Experiment
RNA isolation / purification Cells - immortalized HaCaT
Product
HiPurA™ Total RNA Miniprep Purification Kit from HiMEDIA
Manufacturer
HiMEDIA

Protocol tips

Protocol tips
Extraction of total RNA from cells (control and experimental) with 105 cells/mL concentration was done using HiPure kit (HiMedia, India), following the instructions of manufacturer.The quantity and quality of the extracted RNA was checked with spectrophotometer at 260 and 280 nmand by agarose gel electrophoresis.

Publication protocol

Extraction of total RNA from cells (control and experimental) with 105 cells/mL concentration was done using HiPure kit (HiMedia, India), following the instructions of manufacturer.The quantity and quality of the extracted RNA was checked with spectrophotometer at 260 and 280 nmand by agarose gel electrophoresis. Reverse transcription reaction was performed using 1 𝜇g of RNA with high-capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA) in a GeneAmp PCR System2720ThermalCycler (Applied Biosystems, USA) according to the manufacturer’s protocol.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

Download the product protocol from HiMEDIA for HiPurA™ Total RNA Miniprep Purification Kit below.

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