Isolate II RNA Mini Kit

RNA isolation / purification Cells - immortalized HCA2

Experiment
RNA isolation / purification Cells - immortalized HCA2
Product
Isolate II RNA Mini Kit from Bioline
Manufacturer
Bioline

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 1x10^6 cells

Publication protocol

otal RNA was prepared using the Isolate II Rna Mini Kit (Bioline). 255 – 500 ng of RNA was reverse transcribed into cDNA using a kit (Applied Biosystems). qRT-PCR reactions were performed as described [1] using the Universal Probe Library system (Roche) and a SENSIFast Probe kit (Bioline). Expression of tubulin was used to normalize the expression of CT values

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Discussion

Discussion

1 year ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized HCA2 using Isolate II RNA Mini Kit from Bioline.

Paper title
Unmasking Transcriptional Heterogeneity in Senescent Cells
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Manufacturer protocol

Download the product protocol from Bioline for Isolate II RNA Mini Kit below.

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