Isolate II RNA Mini Kit

RNA isolation / purification Cells - immortalized HCA2

Experiment
RNA isolation / purification Cells - immortalized HCA2
Product
Isolate II RNA Mini Kit from Bioline
Manufacturer
Bioline

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 1x10^6 cells

Publication protocol

otal RNA was prepared using the Isolate II Rna Mini Kit (Bioline). 255 – 500 ng of RNA was reverse transcribed into cDNA using a kit (Applied Biosystems). qRT-PCR reactions were performed as described [1] using the Universal Probe Library system (Roche) and a SENSIFast Probe kit (Bioline). Expression of tubulin was used to normalize the expression of CT values

Full paper   Login or join for free to view the full paper.

Reviews

Isolate II RNA Mini Kit from Bioline has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized HCA2 using Isolate II RNA Mini Kit from Bioline.

Paper title
Unmasking Transcriptional Heterogeneity in Senescent Cells
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Bioline for Isolate II RNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized HCA2 using Isolate II RNA Mini Kit from Bioline. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms