MagMAX™ Total Nucleic Acid Isolation Kit
RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis
Protocol tips
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| Kit relies on bead-beating and magnetism, so adequate lab equipment is required, check the manual for required materials. |
Publication protocol
a commercial kit and protocol was followed (MagMAX™ Total Nucleic Acid Isolation Kit, 96 well plate format, Thermo Fisher Scientific).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Paul G. Macon
Some help with RNA isolation using Trizol
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
Papers
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Manufacturer protocol
Download the product protocol from Thermo Fisher Scientific for MagMAX™ Total Nucleic Acid Isolation Kit below.
Download manufacturer protocol
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