TRIzol Reagent

RNA isolation / purification Cells - immortalized HL-60

Experiment
RNA isolation / purification Cells - immortalized HL-60
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was extracted from cell lines Trizol (Invitrogen Life Technologies), according to the manufacturer’s instructions. Next, the first strand cDNA was synthesized from RNA by reverse transcription using PrimeScript™ RT reagent Kit (TaKaRa Bio Group, Dalian, China), according to the manufacturer’s instructions. The primers are listed in Table 1. Real-time PCR was performed using the CXF96 (BioRad, CA, USA) system and a SyberGreen II real-time PCR kit (TaKaRa). The PCR reaction conditions were as follows: denaturation at 95°C for 3 min, followed by 39 cycles at 95°C (30 s), 59°C (30 s) and 72°C (30 s), and a final extension at 72°C for 5 min.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized HL-60 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
HA117 endows HL60 cells with a stem-like signature by inhibiting the degradation of DNMT1 via its ability to down-regulate expression of the GGL domain of RGS6
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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Videos

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