TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was extracted from cell lines Trizol (Invitrogen Life Technologies), according to the manufacturer’s instructions. Next, the first strand cDNA was synthesized from RNA by reverse transcription using PrimeScript™ RT reagent Kit (TaKaRa Bio Group, Dalian, China), according to the manufacturer’s instructions. The primers are listed in Table 1. Real-time PCR was performed using the CXF96 (BioRad, CA, USA) system and a SyberGreen II real-time PCR kit (TaKaRa). The PCR reaction conditions were as follows: denaturation at 95°C for 3 min, followed by 39 cycles at 95°C (30 s), 59°C (30 s) and 72°C (30 s), and a final extension at 72°C for 5 min.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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