PureHelix™ RNA Extraction Solution

RNA isolation / purification Cells - immortalized HT-1080

Experiment
RNA isolation / purification Cells - immortalized HT-1080
Product
PureHelix™ RNA Extraction Solution from NanoHelix
Manufacturer
NanoHelix

Protocol tips

Protocol tips
Total RNA was extracted using PureHelix™ RNA extraction solution and reverse transcribed to cDNA using HelixCript™ 1st Strand cDNA Synthesis kit (NanoHelix Co., Daejeon, Korea).

Publication protocol

Total RNA was extracted using PureHelix™ RNA extraction solution and reverse transcribed to cDNA using HelixCript™ 1st Strand cDNA Synthesis kit (NanoHelix Co., Daejeon, Korea). cDNA aliquots corresponding to 1 μg RNA were analyzed by semi-quantitative PCR using the following primers; hMMP-9, 5′-TCTTCCCTGGAGACTGAGAA-3′ and 5′-GGCAAGTCTTCCGAGTAGTTT-3′; GAPDH, 5′-TCATGACCACAGTCCATGCC-3′ and 5′-TCCACCACCCTGTTGCTGTA-3′.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized HT-1080 using PureHelix™ RNA Extraction Solution from NanoHelix.

Paper title
Suppression of the invasive potential of highly malignant tumor cells by KIOM-C, a novel herbal medicine, via inhibition of NF-κB activation and MMP-9 expression.
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Manufacturer protocol

Download the product protocol from NanoHelix for PureHelix™ RNA Extraction Solution below.

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