PureHelix™ RNA Extraction Solution

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	Total RNA was extracted using PureHelix™ RNA extraction solution and reverse transcribed to cDNA using HelixCript™ 1st Strand cDNA Synthesis kit (NanoHelix Co., Daejeon, Korea).

Protocol tips
Total RNA was extracted using PureHelix™ RNA extraction solution and reverse transcribed to cDNA using HelixCript™ 1st Strand cDNA Synthesis kit (NanoHelix Co., Daejeon, Korea).

Publication protocol

Total RNA was extracted using PureHelix™ RNA extraction solution and reverse transcribed to cDNA using HelixCript™ 1st Strand cDNA Synthesis kit (NanoHelix Co., Daejeon, Korea). cDNA aliquots corresponding to 1 μg RNA were analyzed by semi-quantitative PCR using the following primers; hMMP-9, 5′-TCTTCCCTGGAGACTGAGAA-3′ and 5′-GGCAAGTCTTCCGAGTAGTTT-3′; GAPDH, 5′-TCATGACCACAGTCCATGCC-3′ and 5′-TCCACCACCCTGTTGCTGTA-3′.

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Manufacturer protocol

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