SV Total RNA Isolation System

RNA isolation / purification Cells - immortalized HT-29

Experiment
RNA isolation / purification Cells - immortalized HT-29
Product
SV Total RNA Isolation System from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Publication protocol

The expression levels of selected genes were validated by qRT-PCR. Thus, HT-29 cells were either untreated (vehicle control, 0.2% DMSO), or treated with cyclopamine, GANT61, purmorphamine, butyrate, LPS, EGF, or IFN-γ, for 24 hr at 37°C, dissolved in DMSO-containing medium. Total RNA isolation from cultured cells was performed using SV Total RNA isolation systems (Promega, Madison, WI, USA), following the manufacturer’s protocol. The Nanodrop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE, USA) was used for quantifying and determining the RNA purity of samples. Equal amounts of total RNA were reverse transcribed using RT2 First Strand Kit (SABiosciences, Frederick, MD, USA).

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized HT-29 using SV Total RNA Isolation System from Promega.

Paper title
Hedgehog Pathway Signaling Regulates Human Colon Carcinoma HT-29 Epithelial Cell Line Apoptosis and Cytokine Secretion
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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

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Videos

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