SV Total RNA Isolation System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Protocol tips
For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Publication protocol

The expression levels of selected genes were validated by qRT-PCR. Thus, HT-29 cells were either untreated (vehicle control, 0.2% DMSO), or treated with cyclopamine, GANT61, purmorphamine, butyrate, LPS, EGF, or IFN-γ, for 24 hr at 37°C, dissolved in DMSO-containing medium. Total RNA isolation from cultured cells was performed using SV Total RNA isolation systems (Promega, Madison, WI, USA), following the manufacturer’s protocol. The Nanodrop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE, USA) was used for quantifying and determining the RNA purity of samples. Equal amounts of total RNA were reverse transcribed using RT2 First Strand Kit (SABiosciences, Frederick, MD, USA).

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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

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