TRIzol Reagent

RNA isolation / purification Cells - immortalized JY

Experiment
RNA isolation / purification Cells - immortalized JY
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was isolated from Hs 611.T and Akata cells using TRIzol reagent (Life Technologies, catalog number 15596-018) according to the vendor's protocol. Nuclear and cytoplasmic RNA was isolated using a cytoplasmic and nuclear RNA purification kit (Norgen Biotek Corp., catalog number 21000) according to the vendor's protocol. All RNA preparations were subjected to DNase treatment using the DNA-free kit (Life Technologies, catalog number AM1906). Standard reverse transcription was performed using an iScript cDNA synthesis kit (Bio-Rad, catalog number 170-8891) according to the vendor's protocol.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized JY using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
High-Throughput RNA Sequencing-Based Virome Analysis of 50 Lymphoma Cell Lines from the Cancer Cell Line Encyclopedia Project
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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