TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was isolated from Hs 611.T and Akata cells using TRIzol reagent (Life Technologies, catalog number 15596-018) according to the vendor's protocol. Nuclear and cytoplasmic RNA was isolated using a cytoplasmic and nuclear RNA purification kit (Norgen Biotek Corp., catalog number 21000) according to the vendor's protocol. All RNA preparations were subjected to DNase treatment using the DNA-free kit (Life Technologies, catalog number AM1906). Standard reverse transcription was performed using an iScript cDNA synthesis kit (Bio-Rad, catalog number 170-8891) according to the vendor's protocol.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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