RNeasy Mini Kit

RNA isolation / purification Cells - immortalized K-562

Experiment
RNA isolation / purification Cells - immortalized K-562
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min and Proteinase-K treatment.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

For total RNA isolation, RNeasy Mini Kit (Qiagen, Hilden, Germany) was used and the isolation was made according to the manufacturer's instructions. Also RNA from CML cell lines K562, BV-173 and CML-T1 was isolated using the same kit. To remove possible DNA contamination, a DNase free set (Qiagen) was used as instructed by the manufacturer. cDNA was synthesized from up to 0,5 µg RNA with iScript cDNA synthesis kit according to the manufacturer's instructions (BioRad, Hercules, CA, USA).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized K-562 using RNeasy Mini Kit from Qiagen.

Paper title
Increased Level of Myeloid-Derived Suppressor Cells, Programmed Death Receptor Ligand 1/Programmed Death Receptor 1, and Soluble CD25 in Sokal High Risk Chronic Myeloid Leukemia
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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