QIAamp DNA Blood Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction
DNA samples used for assay development and validation were from breast cancer cell lines MDA-MB-231 and SK-BR-3 (obtained from ATCC, Manassas, VA, USA) and human genomic DNA (Roche Applied Science, West Sussex, UK), comprising a pool of ∼100 blood donors from both sexes.



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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Blood Mini Kit below.

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