Mini Total RNA Kit

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	total RNA was isolated using a Total RNA minikit (catalog number IB147323; IBI). RNA was quantified, and equal amounts of total RNA were treated with DNase I (M6101; Promega)

Protocol tips
total RNA was isolated using a Total RNA minikit (catalog number IB147323; IBI). RNA was quantified, and equal amounts of total RNA were treated with DNase I (M6101; Promega)

Publication protocol

For DNA analysis, total DNA was isolated using a Genomic DNA minikit (catalog number IB47202; IBI). DNA was amplified by PCR using a GoTaq Flexi system (M8295; Promega). For RNA analysis, total RNA was isolated using a Total RNA minikit (catalog number IB147323; IBI). RNA was quantified, and equal amounts of total RNA were treated with DNase I (M6101; Promega) and used for reverse transcription-PCR (RT-PCR; 35 to 40 cycles) using a Superscript III one-step RT-PCR system (catalog number 12574-026; Invitrogen). Primer pairs for IE1 that span the intron between exon 2 and exon 3 (29), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (60), and LUNA (latency unique nuclear antigen) (61) have been described previously. PCR products were separated on 1.5% agarose gels, and bands were imaged and quantified using an Odyssey Fc imager with Image Studio, version 2.1.10, software (Li-Cor).

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