Mini Total RNA Kit

RNA isolation / purification Cells - immortalized KG-1

Experiment
RNA isolation / purification Cells - immortalized KG-1
Product
Mini Total RNA Kit from IBI Scientific
Manufacturer
IBI Scientific

Protocol tips

Protocol tips
total RNA was isolated using a Total RNA minikit (catalog number IB147323; IBI). RNA was quantified, and equal amounts of total RNA were treated with DNase I (M6101; Promega)

Publication protocol

For DNA analysis, total DNA was isolated using a Genomic DNA minikit (catalog number IB47202; IBI). DNA was amplified by PCR using a GoTaq Flexi system (M8295; Promega). For RNA analysis, total RNA was isolated using a Total RNA minikit (catalog number IB147323; IBI). RNA was quantified, and equal amounts of total RNA were treated with DNase I (M6101; Promega) and used for reverse transcription-PCR (RT-PCR; 35 to 40 cycles) using a Superscript III one-step RT-PCR system (catalog number 12574-026; Invitrogen). Primer pairs for IE1 that span the intron between exon 2 and exon 3 (29), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (60), and LUNA (latency unique nuclear antigen) (61) have been described previously. PCR products were separated on 1.5% agarose gels, and bands were imaged and quantified using an Odyssey Fc imager with Image Studio, version 2.1.10, software (Li-Cor).

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized KG-1 using Mini Total RNA Kit from IBI Scientific.

Paper title
Myeloblastic Cell Lines Mimic Some but Not All Aspects of Human Cytomegalovirus Experimental Latency Defined in Primary CD34 Cell Populations
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Manufacturer protocol

Download the product protocol from IBI Scientific for Mini Total RNA Kit below.

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