RNeasy Micro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.		- Always blank the spectrophotometer with your elution buffer. - When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer. - When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

KU812 cells transduced with a random (RDM) short hairpin RNA (shRNA) or a CD25 shRNA were subjected to RNA isolation and gene array analysis using Affymetrix technology.

Total RNA was extracted from CML cells using RNeasy Micro-Kit (Qiagen) and used (150 ng total RNA) for Gene Chip analyses. Preparation of terminal-labeled cRNA, hybridization to genome-wide human Gene 2.0 ST Arrays (Affymetrix, Santa Clara, CA, USA) and scanning of arrays were carried out according to the manufacturer's protocols (https://www.affymetrix.com). Robust Multichip Average (RMA) signal extraction and normalization were performed using GeneChip Expression Console Version 1.3.1. Differences in mRNA expression levels were calculated as normalized fluorescence signal ratio of KU812 CD25 shRNA cells versus KU812 RDM shRNA cells.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

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