Direct-zol RNA Kits

RNA isolation / purification Cells - immortalized LNCaP

Experiment
RNA isolation / purification Cells - immortalized LNCaP
Product
Direct-zol RNA Kits from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Upstream tips
- Highly effective when processing various sample types in TRI Reagent®, TRIzol®

Publication protocol

Total RNA was extracted from collected cell pellets using Direct-sol MiniPrep kit (Zymo Research) according to the manufacturer's instructions. For each sample, RNA quantification and quality control were assessed by NanoDrop 2000c measurements (Thermo Scientific). A final amount of 5 ng of the template RNA was required for first-strand cDNA synthesis of miRNAs with the Universal cDNA Synthesis kit II (Exiqon).

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

Download the product protocol from Zymo Research for Direct-zol RNA Kits below.

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Videos

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