RNeasy Plus Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total mRNA was isolated from tumour cells using RNeasy Plus Kit (Qiagen, Hilden, Germany) and RNA was reverse transcribed into cDNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems), following the instructions from the manufacturers. Real-time quantitative RT-PCR was carried out with specific TaqMan Gene Expression assays. Relative mRNA expression was calculated by the 2−ΔΔCt method after normalizing expression levels of investigated mRNA to ribosomal protein lateral stalk subunit P0 (RPLP0) mRNA.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

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