RNeasy Plus Mini Kit

RNA isolation / purification Cells - immortalized Ma-Mel

Experiment
RNA isolation / purification Cells - immortalized Ma-Mel
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total mRNA was isolated from tumour cells using RNeasy Plus Kit (Qiagen, Hilden, Germany) and RNA was reverse transcribed into cDNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems), following the instructions from the manufacturers. Real-time quantitative RT-PCR was carried out with specific TaqMan Gene Expression assays. Relative mRNA expression was calculated by the 2−ΔΔCt method after normalizing expression levels of investigated mRNA to ribosomal protein lateral stalk subunit P0 (RPLP0) mRNA.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized Ma-Mel using RNeasy Plus Mini Kit from Qiagen.

Paper title
Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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