QIAamp DNA Mini Kit

DNA isolation / purification Cells - Primary cells Cyst-derived kidney epithelial cells

Experiment

DNA isolation / purification Cells - Primary cells Cyst-derived kidney epithelial cells

Product

QIAamp DNA Mini Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

For detection of SV40 DNA integration into the cell genome, DNA was isolated, using a commercial kit (QIAmp DNA mini kit, Qiagen). SV40 DNA was amplified by PCR, using specific oligonucleotide primers (Table 2).

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Reviews

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Discussion

5 years ago

Author: R. Verma India

DNA isolation column clogged

During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Cells - Primary cells Cyst-derived kidney epithelial cells using QIAamp DNA Mini Kit from Qiagen.

Paper title

Immortalized epithelial cells from human autosomal dominant polycystic kidney cysts.

Full paper

Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

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Videos

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