High Pure RNA Isolation Kit

RNA isolation / purification Cells - immortalized MCF 10A

Experiment
RNA isolation / purification Cells - immortalized MCF 10A
Product
High Pure RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
- To increase nucleic yield or purity store all buffer at +15C to +25C.
Protocol tips
- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately.

Publication protocol

Total RNA isolation was performed using the High Pure RNA Isolation Kit (Roche Diagnostics, Basel, Switzerland). Reverse transcription was performed using 1 μg of RNA template with the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized MCF 10A using High Pure RNA Isolation Kit from Roche Lifesciences.

Paper title
Circadian Regulation of Benzo[a]Pyrene Metabolism and DNA Adduct Formation in Breast Cells and the Mouse Mammary Gland
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

Download PDF Download manufacturer protocol

Videos

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