ReliaPrep™ RNA Miniprep Systems

RNA isolation / purification Cells - immortalized MDA-MB-231

Experiment
RNA isolation / purification Cells - immortalized MDA-MB-231
Product
ReliaPrep™ RNA Miniprep Systems from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
- Before beginning the ReliaPrep™ RNA Cell Miniprep System protocol, four solutions must be prepared.

Publication protocol

The four cell lines used in this study were purchased from ATCC (American Type Culture Collection, Manassas, VA, USA). All cell culture reagents were purchased from Life Technologies (Grand Island, NY, USA), except for charcoal/dextran stripped serum, which was purchased from Atlanta Biologicals (Flowery Branch, GA, USA). 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 were purchased from Sigma (St. Louis, MO, USA) and diluted to 1 mM with ethanol. The CellTiter-Glo, ReliaPrep RNA Cell Miniprep System and GoTaq 1-step RT-qPCR systems were purchased from Promega (Madison, WI, USA). QuantiTect Primer Assays were purchased from Qiagen (Germantown, MD, USA).

Full paper   Login or join for free to view the full paper.

Reviews

ReliaPrep™ RNA Miniprep Systems from Promega has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized MDA-MB-231 using ReliaPrep™ RNA Miniprep Systems from Promega.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Promega for ReliaPrep™ RNA Miniprep Systems below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized MDA-MB-231 using ReliaPrep™ RNA Miniprep Systems from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms