ReliaPrep™ RNA Miniprep Systems

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Before beginning the ReliaPrep™ RNA Cell Miniprep System protocol, four solutions must be prepared.

Upstream tips
- Before beginning the ReliaPrep™ RNA Cell Miniprep System protocol, four solutions must be prepared.

Publication protocol

The four cell lines used in this study were purchased from ATCC (American Type Culture Collection, Manassas, VA, USA). All cell culture reagents were purchased from Life Technologies (Grand Island, NY, USA), except for charcoal/dextran stripped serum, which was purchased from Atlanta Biologicals (Flowery Branch, GA, USA). 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 were purchased from Sigma (St. Louis, MO, USA) and diluted to 1 mM with ethanol. The CellTiter-Glo, ReliaPrep RNA Cell Miniprep System and GoTaq 1-step RT-qPCR systems were purchased from Promega (Madison, WI, USA). QuantiTect Primer Assays were purchased from Qiagen (Germantown, MD, USA).

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Manufacturer protocol

Download the product protocol from Promega for ReliaPrep™ RNA Miniprep Systems below.

Download manufacturer protocol

Videos

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