PureLink™ RNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required.	- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit.

Protocol tips
- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required.
Downstream tips
- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit.

Publication protocol

High-quality and purified RNA was isolated from in situ breast carcinoma tumors or in vitro BC cell lines using a PureLink RNA Mini Kit (Thermo Fisher Scientific) according to manufacturer’ recommendation. A volume of 10 µg RNA of each sample was then retrotranscribed into complimentary DNA (cDNA) using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA) according to manufacturer’ recommendation. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted on a 7900HT Fast Real-Time PCR system (Applied Biosystems). For miR-409-5p measurement, a TaqMan microRNA Assay (Applied Biosystems, USA) was performed and a non-coding RNA SNORD66 was used as loading control. For RSU1 gene measurement, a Brilliant SYBR-Green qRT–PCR kit (Stratagene, USA) was used, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. Relative fold changes of gene expressions were then quantified using the 2−ΔΔCt method.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ RNA Mini Kit below.

Download manufacturer protocol

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