PureLink™ RNA Mini Kit

RNA isolation / purification Cells - immortalized MDA-MB-231

Experiment
RNA isolation / purification Cells - immortalized MDA-MB-231
Product
PureLink™ RNA Mini Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required.
Downstream tips
- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit.

Publication protocol

High-quality and purified RNA was isolated from in situ breast carcinoma tumors or in vitro BC cell lines using a PureLink RNA Mini Kit (Thermo Fisher Scientific) according to manufacturer’ recommendation. A volume of 10 µg RNA of each sample was then retrotranscribed into complimentary DNA (cDNA) using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA) according to manufacturer’ recommendation. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted on a 7900HT Fast Real-Time PCR system (Applied Biosystems). For miR-409-5p measurement, a TaqMan microRNA Assay (Applied Biosystems, USA) was performed and a non-coding RNA SNORD66 was used as loading control. For RSU1 gene measurement, a Brilliant SYBR-Green qRT–PCR kit (Stratagene, USA) was used, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. Relative fold changes of gene expressions were then quantified using the 2−ΔΔCt method.

Full paper   Login or join for free to view the full paper.

Reviews

PureLink™ RNA Mini Kit from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized MDA-MB-231 using PureLink™ RNA Mini Kit from Thermo Fisher Scientific.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ RNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized MDA-MB-231 using PureLink™ RNA Mini Kit from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms