Qubit dsDNA HS Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not label the side of the tube as this could interfere with the sample read.

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

RNA-seq library construction and cDNA sequencing.
Size distribution and concentration of single-cell cDNA was assessed by high-throughput capillary gelelectrophoresis (Fragment Analyzer, Advanced Analytical). Sequencing libraries were constructed in 96-well plates using the Illumina Nextera XT DNA Sample Preparation Kit according to the protocol supplied by Fluidigm and as described previously (15). Libraries were quantified by Agilent Bioanalyzer using High Sensitivity DNA Analysis Kit as well as fluorometrically using Qubit dsDNA HS Assay Kits and a Qubit 2.0 Fluorometer (Invitrogen, Life Technologies). Up to 96 single-cell libraries were pooled, and each cell was sequenced 100 bp paired-end on Illumina HiSEq. 2500 to a depth of 2–5 million reads. Base-calling, adaptor trimming, and de-multiplexing were performed as described (33, 34).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit dsDNA HS Assay Kit below.

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