RNeasy FFPE Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	All 4 methods successfully extracted RNA (determined as generating a measurable signal using the Qubit) from as little as one 5 μm section, with geometric mean RNA yields greater than 100 ng (Table 1/Fig. 1). The Qiagen RNeasy FFPE extraction kit gave a superior geometric mean RNA yield of 398 ng

Protocol tips
All 4 methods successfully extracted RNA (determined as generating a measurable signal using the Qubit) from as little as one 5 μm section, with geometric mean RNA yields greater than 100 ng (Table 1/Fig. 1). The Qiagen RNeasy FFPE extraction kit gave a superior geometric mean RNA yield of 398 ng

Publication protocol

All 4 methods successfully extracted RNA (determined as generating a measurable signal using the Qubit) from as little as one 5 μm section, with geometric mean RNA yields greater than 100 ng (Table 1/Fig. 1). The Qiagen RNeasy FFPE extraction kit gave a superior geometric mean RNA yield of 398 ng; however, the difference in the performance of the RNeasy single RNA extraction and the AllPrep DNA/RNA FFPE kit was not significant (P-value 0.16). Both Qiagen RNA extraction kits significantly outperformed the Artcturus paradise plus FFPE RNA isolation kit and the automated Maxwell 16 LEV RNA FFPE Kit. The RNeasy FFPE Kit gave geometric mean RNA yields 166 ng greater than the Maxwell 16 and 200 ng greater than the Artcturus paradise plus FFPE RNA isolation kit.



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Manufacturer protocol

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