ReliaPrep™ RNA Miniprep Systems

RNA isolation / purification Cells - immortalized MDA-MB-468

Experiment
RNA isolation / purification Cells - immortalized MDA-MB-468
Product
ReliaPrep™ RNA Miniprep Systems from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
- Before beginning the ReliaPrep™ RNA Cell Miniprep System protocol, four solutions must be prepared.

Publication protocol

The four cell lines used in this study were purchased from ATCC (American Type Culture Collection, Manassas, VA, USA). All cell culture reagents were purchased from Life Technologies (Grand Island, NY, USA), except for charcoal/dextran stripped serum, which was purchased from Atlanta Biologicals (Flowery Branch, GA, USA). 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 were purchased from Sigma (St. Louis, MO, USA) and diluted to 1 mM with ethanol. The CellTiter-Glo, ReliaPrep RNA Cell Miniprep System and GoTaq 1-step RT-qPCR systems were purchased from Promega (Madison, WI, USA). QuantiTect Primer Assays were purchased from Qiagen (Germantown, MD, USA).

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized MDA-MB-468 using ReliaPrep™ RNA Miniprep Systems from Promega.

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Manufacturer protocol

Download the product protocol from Promega for ReliaPrep™ RNA Miniprep Systems below.

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Videos

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