TRI Reagent™ Solution

RNA isolation / purification Cells - immortalized MG63

Experiment
RNA isolation / purification Cells - immortalized MG63
Product
TRI Reagent™ Solution from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- Carefully watch how much TRI reagent should be added to your samples.

Publication protocol

After 7, 14, or 21 days of culturing, total RNA was extracted from the samples using TriReagent according to the manufacturer's instructions. In brief, ice-cold Tri Reagent (1 mL) was added to the samples, and they were incubated for 5 min at room temperature. Then, 1-bromo-3-chloropropane (BCP; 0.1 mL) was added, and they were incubated for a further 15 min at room temperature. The samples were centrifuged at 12,000× g for 15 min at 4°C, and the upper aqueous phase that contained the RNA was collected. The RNA was precipitated using isopropanol and centrifuged at 12,000× g for 10 min at 4°C. Following precipitation, 1 mL of 75% ethanol was added into the RNA pellet, followed by centrifugation at 7500× g for 5 min, and the resulting pellet was air dried. The RNA pellet was dissolved in DEPC water. The concentration and purity of each sample were assessed by absorbance at 260 nm and by the 260/280 nm ratio, respectively.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized MG63 using TRI Reagent™ Solution from Thermo Fisher Scientific.

Paper title
MG63 Osteoblast-Like Cells Exhibit Different Behavior when Grown on Electrospun Collagen Matrix versus Electrospun Gelatin Matrix
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRI Reagent™ Solution below.

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