TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was extracted using 1ml if TRIzol reagent (Life technologies) followed by 0.2 ml Chloroform to allow for phase-separation by centrifugation. RNA was precipitated using isopropanol and further washed with 80 % Ethanol and re-suspended in nuclease-free water.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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