Qubit dsDNA HS Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not label the side of the tube as this could interfere with the sample read.

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

Sample preparation
Samples were blinded and labelled 1–8 (figure 1). Laboratories were anonymised (A–M). Samples were shipped at room temperature and stored at 5°C. One sample set was validated at UCL using clinical protocols. DNA was extracted using the QIAamp DNA FFPE tissue kit (Qiagen, Germantown, Maryland, USA). DNA was quantified using Qubit V2.0 (Invitrogen, Carlsbad, USA). BRAF/EGFR screening was performed using Sanger sequencing. Primer sequences are listed in online supplementary table S1. Results were unblinded and confirmed against vendor specifications.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit dsDNA HS Assay Kit below.

Download manufacturer protocol

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