miRNeasy Mini kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step

Protocol tips
- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step

Publication protocol

Total RNA from cells and EV subpopulations was isolated using the miRNAeasy micro kit (Qiagen, Hilden, Germany), according to the manufacturer´s instructions. RNA samples were eluted in 14 μL of RNAse-free water, aliquoted, and stored at -80 °C. The quality and sized of the isolated RNA was measured by capillary electrophoresis using an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). RNA from each sample was denatured at 72 °C for 2 min and loaded into RNA 6000 Nano and Pico total RNA kits (Agilent Technologies) to analyze RNA profile and concentration. The RNA concentration was used as a normalizing loading factor for all EV samples.

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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Mini kit below.

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