TRIzol Reagent

RNA isolation / purification Cells - immortalized PNT2

Experiment
RNA isolation / purification Cells - immortalized PNT2
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

total RNA was extracted with TRIzol (Life Technology) according to the supplier’s protocol. The RNA concentrations were measured using a NanoDrop (ND-1000, Spectrophotometer, Thermo Scientific, Wilmington, DE). For external validation of our results, we analysed a microarray data set from Taylor et al. (16) constituting 110 prostate cancer tissue samples and 28 non-malignant adjacent benign prostate tissue samples (GEO accession number GSE21032)

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Discussion

Discussion

1 year ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized PNT2 using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
miR-145 suppress the androgen receptor in prostate cancer cells and correlates to prostate cancer prognosis.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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Videos

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