RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Viral RNAs were isolated from serum using the QIAamp viral RNA minikit (Qiagen) or from B lymphocytes (CD19) and Raji cell lines using the RNeasy minikit (Qiagen). E1E2 envelope glycoprotein sequences were reverse transcribed (Superscript II; Invitrogen), amplified through two successive nested PCRs, and cloned into a phCMV expression plasmid in fusion with the C-terminal part (18 amino acids) of the HCV core (H77; GenBank accession no. AF009606) encoding sequence that acts as a signal peptide sequence.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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