RNA-Bee

RNA isolation / purification Cells - immortalized SKOV-3

Experiment
RNA isolation / purification Cells - immortalized SKOV-3
Product
RNA-Bee from Amsbio
Manufacturer
Amsbio

Protocol tips

Upstream tips
- Adjust buffers to pH to 6.5 - 7.5 if they have have an acidic pH for better results.
Protocol tips
- An additional wash with 75% ethanol improves 260/280 ratio.

Publication protocol

The NucleoSpin kit (Machery-Nagel) was used according to the manufacturer's protocol to isolate DNA from cells harvested with trypsin and washed with PBS.

For the isolation of total RNA (including microRNAs), exponentially growing cells were directly lysed in culture flasks with RNA-Bee to avoid changes in expression due to harvesting or washing. Total RNA was isolated from lysates as previously described. Three independent isolates of RNA from each line were pooled for mRNA and microRNA expression analysis to decrease possible bias from outlier values.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

Download the product protocol from Amsbio for RNA-Bee below.

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