RNAqueous®-Micro Total RNA Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- DNase treament is provided with the kit, but it not required. It is recommended for RT-PCR applications.

Downstream tips
- DNase treament is provided with the kit, but it not required. It is recommended for RT-PCR applications.

Publication protocol

Total RNA was extracted using the RNAqueous™ Micro Kit (Life Technologies, USA) for nucleic acid extraction from small cell populations. All cell populations of interest were lysed directly on the membranes without prior enzymatic detachment. Isolated RNA was immediately tested on yield, purity and quality using a NanoDrop® ND-1000 (Thermo Scientific, USA) and a BioAnalyzer 2100 (Agilent Technologies, USA) device. Samples were stored at -80°C and only samples with absorption (A) ratios A260/A280 ≥ 1.8 and A260/A230 ≥ 1.5 (NanoDrop®) and RNA Integrity Number (RIN) > 6 (BioAnalyzer) were considered for microarray hybridization.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RNAqueous®-Micro Total RNA Isolation Kit below.

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