SV 96 Total RNA Isolation System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Do not freeze-thaw the DNase more than three times after rehydration.	- For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Upstream tips
- Do not freeze-thaw the DNase more than three times after rehydration.
Protocol tips
- For better lysis, pipet the RNA Lysis Buffer over the bottom of the well.

Publication protocol

Total RNA from primary airway epithelial cells (human and pig), Calu-3 cells, T84 cells, and PK1 cells was isolated by use of the mirVana miRNA isolation kit, TRIzol Reagent (Life Technologies, Carlsbad, CA), or the SV96 Total RNA Isolation System (Promega, Madison, WI), according to the manufacturer's protocol. Total RNA was tested for quality on an Agilent model 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Only samples with an RNA integrity number over 7.0 were selected for downstream processing.

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Manufacturer protocol

Download the product protocol from Promega for SV 96 Total RNA Isolation System below.

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