connexin 43 ShRNA

Protocol tips

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	Add 1:1 ratio of DNA to diluted Lipofectamine® 2000 Reagent and Incubate for 5 minutes at room temperature. Incubate cells for 2 days at 37°C then analyze transfected cells

Protocol tips
Add 1:1 ratio of DNA to diluted Lipofectamine® 2000 Reagent and Incubate for 5 minutes at room temperature. Incubate cells for 2 days at 37°C then analyze transfected cells

Publication protocol

The human dopaminergic SH-SY5Y cells cultured in DMEM were supplemented with 10% (v/v) inactivated FBS and 100 U/mL penicillin/streptomycin. These cells were maintained in 37 °C incubator supplied with 5% CO2 and 95% humidified air. Connexin 43 shRNA plasmid and control shRNA plasmid were obtained from Santa Cruz Biotechnology. They were diluted in nuclease free water to a final concentration of 10 µM. Plasmids (pcDNA 3.1-Cx43 recombinant vector, Cx43 shRNA plasmid) were transfected into SH-SY5Y cells at a final total concentration of 15 nM using Transfection Reagent (Lipofectamine 2000 (Invirogen, Carlsbad, CA, USA)). Subsequent measurements were performed at 48 h post transfection.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for connexin 43 ShRNA below.

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