RNeasy Plus Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA from THP-1 monocytes/macrophages was extracted using the RNeasy Plus Mini kit (Qiagen). The RNA was quantified using Thermo Bio-Mate3 and diluted into 1mg/mL. Total RNA (0.5 𝜇g) was processed directly to cDNA synthesis using the oligo (dT) 15 primer and SuperScript II reverse transcriptase reagents (TaKaRa, Dalian, China) according to
the manufacturer’s protocol.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download manufacturer protocol

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