RNeasy Plus Mini Kit

RNA isolation / purification Cells - immortalized THP-1

Experiment
RNA isolation / purification Cells - immortalized THP-1
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA from THP-1 monocytes/macrophages was extracted using the RNeasy Plus Mini kit (Qiagen). The RNA was quantified using Thermo Bio-Mate3 and diluted into 1mg/mL. Total RNA (0.5 𝜇g) was processed directly to cDNA synthesis using the oligo (dT) 15 primer and SuperScript II reverse transcriptase reagents (TaKaRa, Dalian, China) according to
the manufacturer’s protocol.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized THP-1 using RNeasy Plus Mini Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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