Genomic DNA Purification Kit

DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

Experiment
DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus
Product
Genomic DNA Purification Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Downstream tips

- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

PCR amplification, sequencing of spa gene and Spa typing
DNA was extracted from all S. aureus strains, which were grown overnight in nutrient agar (Merck/Germany) at 37 °C using the Fermentas DNA kit, according to the manufacturer's instruction (Fermentas, USA). The primers used for amplification of the X region of the spa gene in this study were as follows: spa-1113f (5-TAAAGACGATCCTTCGGTGAGC-3) and spa-1514r (5-CAGCAGTAGTGCCGTTTGCTT-3) [9]. For the characterization of nucleotide sequences based on the strands, the PCR products were run on 1.5% agarose gels, then sent for sequencing (Bioneer, Korea). The spa typing and evaluation of spa types from these S. aureus strains were performed using the spa database website (http://www.ridom.de/spaserver). The BURP algorithm was used to determine spa clonal complexes (CCs) using the StaphType software v. 1.5 (Ridom GmbH, Würzburg, Germany). When the relationship of various spa types was calculated, insertions, deletions and any recombination in repeats were accounted for. Two default parameters are offered by BURP for clustering analysis: (1) spa types that are shorter than “5 repeats” were excluded, and (2) spa types with a maximum number of costs less than or equal to “4 repeats” were clustered into the same group. A cluster consisting of two or more related spa types was regarded as a clonal complex. A singleton was defined as a spa type that was not grouped into a clonal complex.



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Discussion

Discussion

2 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

2 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus using Genomic DNA Purification Kit from Thermo Fisher Scientific.

Paper title
Phylogenetic relationships among Staphylococcus aureus isolated from clinical samples in Mashhad, Iran.
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Genomic DNA Purification Kit below.

Download PDF Download manufacturer protocol

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