TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA from patients’ tissues, serum and cell lines were extracted using Trizol (Invitrogen, USA) according to the manufacturer’s instructions. For qRT-PCR analysis of BIM mRNA expression, the cDNA synthesis was carried out by the SuperScript II (Invitrogen) followed by the PCR performed by using TaqMan Gene Expression Assay (Applied Biosystem, Life Technologies Corporation, USA). GAPDH was used as an internal control. For analysis of miR-24 expression, mature miRNA was detected by using TaqMan microRNA assays that were specific for miR-24 (Applied Biosystem), and U6 snRNA was used as the internal control. The relative expression of BIM and miR-24 was determined using the 2−△△CT analysis method

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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